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Versatility Solution

Purifying peripheral blood mononuclear cells (PBMC), FACS/Flow cytometry and hybridoma technology include multiple centrifugation steps of temperature-sensitive samples in many different consumables. This requires great versatility and an advanced temperature management. In addition, PBMC isolation by Ficoll® gradient centrifugation must be carried out with a minimum of vibration during deceleration (breaking) within reasonable time. Optimized acceleration and deceleration ramps speed up high-yield PBMC isolation up to 88% (see Application Note 372). Refrigerated benchtop Centrifuge 5910 Ri in combination with swing-bucket Rotor S-4xUniversal offer great versatile and an advanced cooling performance for optimal sample protection. The new user management including run records optimally supports GxP documentation.

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Key Benefits

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Increased Efficiency‌

Improved Sample Safety‌

Better Ergonomics‌

Components

Centrifuge 5910 Ri

  • Super versatile multipurpose centrifuge with high capacity for broad range of consumables, refrigerated for temperature sensitive samples.
  • New swing-bucket standard-rotor S-4xUniversal and its unique universal adapters accommodate bottles, plates and conical tubes.
  • Max. capacity: 4 × 1,000 mL/4 × 5 MTP

Universal Adapter 1

  • For 5.0 mL and 15 mL conical tubes, MTP/DWP
  • Max. capacity: 17 x 5/15 mL tubes or 1 x MTP/DWP
  • Max. speed: 4,324 × g (tubes), 3,237 × g (plates)

Rotor S-4xUniversal with buckets

  • Swing-bucket rotor for broad range of high capacity applications in tubes, bottles and plates
  • Aerosol-tight caps optional available.
  • Max. speed: 4,347 × g (4,500 rpm).

Universal Adapter 3

  • For 25 mL and 50 mL conical tubes, 250 mL bottles, MTP
  • Max. capacity: 7 x 25/50 mL tubes, 1 x bottles or MTP
  • Max. speed: 4,324 × g (50 mL tubes), 2,604 × g (plates)

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Recommended Consumables

Key Methods

Consumables

Advantages

Antigen concentration

  • Protein LoBind® Tubes 15 mL, PCR clean
  • Protein LoBind® Tubes 50 mL, PCR clean
  • Conical Tubes 25 mL, screw cap, Starter Pack, PCR clean
  • DWP 96x2000µL White, Protein LoBind® , PCR clean
  • Minimization of unspecific sample loss with LoBind® consumables
  • Safe sample handling due to lid tightness
  • Safe centrifugation with high g-force stability & screw Cap
Hybridoma technology
  • Conical Tubes 50 mL rack, sterile
  • Conical Tubes 15 mL rack, sterile
  • Protein LoBind® Tubes 15 mL, PCR clean
  • Protein LoBind® Tubes 50 mL, PCR clean
  • Conical Tubes 25 mL, SnapTec® , sterile
  • Reduced risk of culture contamination with high grade sterile purity grade
  • Minimization of unspecific sample loss with LoBind® consumables
FACS/Flow cytometry
  • Protein LoBind® Tubes 15 mL, PCR clean
  • Protein LoBind® Tubes 50 mL, PCR clean
  • Optional: Conical Tubes 50 mL, amber, sterile
  • Optional: Conical Tubes 25 mL, screw cap, PCR clean
  • Minimization of sample photodegradation with LoBind® consumables
  • Less degradation of photo-sensitive samples due to amber material
PBMC Isolation
  • Conical Tubes 50 mL rack, sterile
  • Conical Tubes 15 mL rack, sterile
  • Conical Tubes 25 mL, screw cap, sterile
  • Reduced risk of culture contamination with high grade sterile purity grade
  • Safe sample handling due to lid tightness
  • Safe centrifugation with high g-force stability & screw Cap

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Learn about all Centrifuge Solutions for mAb

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Request your individual support

Your own dedicated expert assists you in finding the perfect match for your specific mAb workflow. Get your individual consultation!




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